- MEI Hua;
[Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep's main economic characters. [Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep's Birth Weight(BW), Weaning Litter Weight(WLW), Weaning Litter Staple Fineness(WLSF), One-age Staple Fineness(OASF), One-age Staple Length(OASL), One-age Wool Yield(OAWY), Main Economic Characters of One-age Aohan fine-wool sheep, Two-age Staple Fineness(TASF), Two-age Staple Length(TASL), Two-age Wool Yield(TAWY), Two-age Weight (TAW) after cropping. [Result] The heritability was 0.1-0.7. [Conclusion] The heritability of Aohan fine-wool sheep's main characters was middle-and-high.
2013年01期 v.5 1-2+7页 [查看摘要][在线阅读][下载 100K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：66 ] - ZHANG Lin;HU Bei-xia;LU Mao-yang;YANG Shao-hua;XU Chuan-tian;ZHANG Xiu-mei;
[Objective] Aim to establish a kind of efficient detection method for duck flavivirus(DFV). [Method] The method of nested PCR based on flavivirus universal primers which were designed according to the GeneBank flavivirus gene sequence. [Result] The degenerate universal primers Flav P1-Flav P4 were designed by genome comparison to target NS5 gene conserved area. The system could only amplify flavivirus purpose gene and the sensitivity was 90 copies/μL, higher than the ordinary PCR 1 000 times. Homology and evolutionary analysis showed that duck flavivirus belonged to mosquito-born flavivirus, NTAV group, similar with Tembusu and BYD virus. [Conclusion]Primers of the nested PCR system had good universality and specificity and method had high sensitivity. This system successfully detected flavivirus and clarified the evolution station of DFV.
2013年01期 v.5 3-7页 [查看摘要][在线阅读][下载 283K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：71 ] - LI Tian-ying;LI Qian-yong;HE Yuan;WANG Qing-hua;
[Objective] This paper aimed to provide references for pet dog kidney and urinary track diseases' x-ray radiography diagnosis. [Method] Experimental dogs were gave two kinds of hypotonic medicines (Vitamin K3 and anisodamine) in advance, non-compression hypotonic (IVP) was done and radiography effects of this two hypotonic medicines were compared later in order to finally discuss clinical practical application value of canine (IVP) using hypotonic medicines. [Result] 3.5 mg/kg.bw anisodamine dosage was injected before radiography, 10 minutes after radiography was the best time to take photo, and the development of experimental dog's bilateral renal contour, renal pelvis and front segment of ureter was basically clear. While regarding radiography by 1.6 mg/kg.bw vitamin K3, the best photo was got 10 minutes later after radiography, and the development of experimental dog's bilateral renal contour, renal pelvis and front segment of ureter was clear. [Conclusion] Vitamin K3, as a kind of hypotonic medicine used in pet clinical (IVP), had characteristics of safety, low dosage and better practical radiography effect, was obviously better than anisodamine, and could be properly promoted in pet clinical (IVP).
2013年01期 v.5 8-10页 [查看摘要][在线阅读][下载 201K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：64 ] - LIU Li-na;LUO Qing-ping;CHENG Guo-fu;HU Xue-ying;YANG Jun;ZHANG Lin;SHAO Hua-bin;LI Shao-wen;
[Objective] To isolate three strains of avian leukosis virus subgroup J(ALV-J) , and then amplify and sequence the gp85 gene. [Method] Three strains of ALV-J were isolated from Hubei Province, which were identified by pathological anatomy, DF-1 cell culture and RT-PCR. And then they were named HB1002, HB1003 and HB1009, respectively.[Result] Test sequence analysis showed: the length of gp85 gene was 921 bp, consistent with expect result; the nucleotide homology between the three isolates was in 97.7%-99.7%,the homology of amino acid was in 95.1%-99%. The nucleotide homology between HPRS-103 and the three isolates was in the 94.1%-94.8%;and the nucleotide homology between other ALV-J and the three isolates was in 87.6%-97.3%.The phylogenetic trees analysis showed that the homology of JS09GY6 virus and the three isolates was nearest, in 95.2%-97.3%.[Conclusion] In the test, the three strains of virus which were isolated were ALV-J.
2013年01期 v.5 11-13+52页 [查看摘要][在线阅读][下载 226K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：68 ] - YANG Zhi-kun;DU Dong-dong;WANG Tao;CHANG Wei-shan;
[Objective] The aim was to explore the antibacterial effect of in-vitro combined application of imipenem and ceftriaxone sodium, imipenem and cefotaxime sodium, amikacin and maquinox on E. coli ATCC25922. [Methods] The minimum inhibition concentration of all kinds of the drugs to E. coli ATCC25922 was determined and bacteriostatic effect of imipenem and ceftriaxone sodium, imipenem and cefotaxime sodium, amikacin and maquinox on E. coli ATCC25922 was determined with chessboard microdilution method. [Results] Combined applications of imipenem and ceftriaxone sodium, imipenem and cefotaxime sodium, amikacin and maquinox to E. coli ATCC25922 revealed synergistic effects. [Conclusion] Combined applications of imipenem and ceftriaxone sodium, imipenem and cefotaxime sodium, amikacin and maquinox greatly reduced drug dosage, improved the drug effect, and provided theoretical support for clinical medicine.
2013年01期 v.5 14-15+19页 [查看摘要][在线阅读][下载 107K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：74 ] - WANG Xue-li;BU Ri-e;YANG Ming-xia;XUE Jiang-dong;
[Objective] In order to clone and express the formyltransferase of Brucella abortus in E.coli, purify the expressed protein and detect its immunogenicity. [Methods] A gene recoding formyltransferase 27-35 ku (Wbkc) was amplified from the genomic DNA of Brucella abortus PCR. The amplified fragments were digested with BamH I and Sal I, and then cloned to the vector pET28a. The constructed recombinant plasmid pET28a-Wbkc was transformed to E.coli BL21 and was induced to express the fusion protein. Then the protein was purified by histidine-binding resin column chromatography, and the immunogenicity was detected by Western blot. The PCR product of Wbkc gene was cloned to the vector pET28a, and the recombinant vector was confirmed by colony PCR identification, recombinant vector digested identification and sequencing analysis. [Results] It was successfully expressed in E.coli BL21 as a fusion protein with histidine at the presence of IPTG, and a specific protein band of 29 ku was found when identified by SDS-PAGE. Western blot showed good immunoreactivity of the expressed product. Wbkc was successfully cloned and expressed, and the purified fusion protein had immunogenicity. This study provides a solid foundation for the further study on the function of proteins and brucellosis diagnostic antigens. [Conclusion] Amplification of formyltransferase gene of Brucella abortus and prokaryotic expression of WbkC, indicating the formyltransferase has specific immune reactivity.
2013年01期 v.5 16-19页 [查看摘要][在线阅读][下载 288K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：57 ] - TANG Jing;MA Lei;ZHOU Fang-ye;SONG Shao-hui;LIAO Guo-yang;BAI Wei;WANG Jun;
[Objective] The aim was to select protective agent of HA antigen of influenza virus. [Method] H3N2 and H1N1 subtype influenza virus were added into A-F groups to conduct accelerated test at 37°C and measure HA titer through hemagglutination test. [Result] Hemagglutination titers of H3N2 and H1N1 subtype influenza virus were 20 and 32 in group A (with PBS buffer solution) for 28 d; heat stability of HA antigen proved the best in group F (with BSA, fucose, proclin 300, triton 100); hemagglutination titers of HA antigen of H3N2 and H1N1 subtype virus were 48 and 96 for 28 d. [Conclusion] Components in group F were best in protection on HA antigen, which can be a candidate of protective agent.
2013年01期 v.5 20-21页 [查看摘要][在线阅读][下载 90K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：72 ] - SHI Zhi-hai;WANG Wen-jia;LAN Ya-li;CHEN Chao-xi;
[Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. [Method] Broth microdilution method was used to determine the minimal inhibitory concentration (MIC) of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. [Result] Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern. [Conclusion] The study provided a theoretical basis for the clinical use of drugs and drug resistance surveillance.
2013年01期 v.5 22-23页 [查看摘要][在线阅读][下载 140K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：92 ] - NA Ren-hua;DONG Hong-min;
[Objective]Through study of different diet categories' effects on lactating cows' feces/urine features and nitrogen emission, and in order to reduce and control the nitrogen emission.[Method]12 China Holsteins each with the weight of 514 kg and at the age of 3 to 5 years old are selected for this experiment to carry out test and research on their feces/urine features and nitrogen emission. The cows are divided into 3 groups which are fed with Diet A which takes dry corn straw as forage with forage to concentrate ratio of 40∶ 60, Diet B and Diet C (both take corn silage as forage) with forage to concentrate ratio of 40∶ 60 and 60∶ 40 respectively. [Result]The results show that diet categories have certain effects on dairy cows' output of feces and urine. Feces output of Diet A (straw type) is more than that of Diet B (silage type with low forage to concentrate ratio) which is more than that of Diet C (silage type with high forage to concentrate ratio). Feces output of Diet A group exceeds that of Diet B and Diet C groups by 6.10% and 10.08% respectively. Feces output of Diet B exceeds that of Diet C by 3.75%. The difference is insignificant(P﹥0.05). In terms of urine output of these three diet groups, Diet C is more than that of Diet B which is more than that of Diet A. Urine output of Diet C exceeds that of Diet B and Diet A by 22.75% and 56.30% respectively. Urine output of Diet B exceeds that of Diet A by 27.33%. The difference is significant(P﹤0.05). At the same time, diet categories have significant effects on dairy cow's nitrogen balance. Straw-typed diet's milk nitrogen and urinary nitrogen contents as well as nitrogen digestibility are far lower than those of silage-typed diet (P﹤0.01). Under conditions of taking corn silage as forage, as diet's concentrate refines, nitrogen digestibility and urinary nitrogen both increase remarkably (P﹤0.01) while fecal nitrogen and milk nitrogen's increase is not obvious (P﹥0.05). [Conclusion]Moreover, it shows that Straw-typed diet's absorbed nitrogen is mainly discharged through feces. When corn silage is taken as forage, as diet's concentrate refines, proportion of fecal nitrogen reduces while that of urinary nitrogen increases, and fecal nitrogen and urinary nitrogen's proportions in total feces/urine nitrogen are basically the same.
2013年01期 v.5 24-27+31页 [查看摘要][在线阅读][下载 171K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：57 ] - ZHANG Xue-yu;SU Yi-jun;LI Guo-hui;TU Yun-jie;JIANG Yong-jun;YANG Fu-sheng;
[Objective] The aim was to explore the classification basis of Chinese local chicken for providing theoretical basis of giving full play to the genetic potential, heterosis, new ideas and methods of new varieties and strains breeding, identification and evaluation. [Method] The multivariate statistical analysis of laying performance and production area ecology of 11 kind of chicken was done by principal component analysis and cluster analysis. [Result] The cluster analysis of 4 egg laying performance indexs indicated that 11 kinds of chicken can be roughly classified into large chicken and small chicken; 10 indexs of multivariate statistical analysis indicated that 11 kinds of chicken can be classified into high altitude type and low altitude type when first 3 eigenvalues were selected as 3 principal component by principal component analysis(94.99% of the total amount of information), and similarity coefficient was computed according to first three principal component values of each kind and then cluster analysis was done with nearest neighbor way. [Conclusion] Ecological factor was also an important aspect of assortment.
2013年01期 v.5 28-31页 [查看摘要][在线阅读][下载 197K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：74 ] - TANG Jian-yang;ZHENG Xue-fang;LIU Bo;LAN Jiang-lin;LU Shu-xian;HU Gui-ping;
[Objective] This experiment aimed to compare piglets behavior under microbial fermentation bed breeding mode and traditional rearing mode. [Method] Pig behavior's statistical analysis under both microbial fermentation bed pig breeding mode and traditional rearing mode was selected to study the effect of different breeding environment on pig's growth [Result]The results showed that the duration of piglets lying and sleeping behavior accounted for the highest proportion of tested behavior, up to more than 70%,followed by feeding and drinking which reached 15.96% (under microbial fermentation bed breeding mode) and 9.33% (under traditional rearing mode) respectively. Compared with traditional intensive rearing mode, piglet exploratory behavior's occurrence probability and duration increased significantly, by 28.62% and 12.21% respectively. Fighting behavior's occurrence probability and duration decreased significantly, by 49.83% and 91.26% respectively. Nutrient index and health index were introduced to evaluate piglet's general heath condition under microbial fermentation bed breeding mode, and results indicated that the health index of piglets under microbial fermentation bed breeding mode had slight difference from that under traditional mode, namely 10.83 and 10.03 respectively. But health index of piglets under microbial fermentation bed breeding mode was 245.12, much higher than 21.96 under traditional mode. [Conclusion] Piglets would be healthier under microbial fermentation bed breeding mode.
2013年01期 v.5 32-36页 [查看摘要][在线阅读][下载 170K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：148 ] - ZHANG Yue-ping;MAO Wei;LI Wen-xi;
Based on surveying the conditions of large-scale farms and commercial manure in the each county of Yangzhou city, the situations and problems for utilization of livestock manure resources were grasped. After an analysis of the potential value of livestock manure, the suggestion and strategy for utilization of livestock manure resources were proposed based on the actual conditions in Yangzhou city.
2013年01期 v.5 37-40+49页 [查看摘要][在线阅读][下载 170K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：73 ] - CHANG Zhao-feng;HAN Fu-gui;ZHONG Sheng-nian;WANG Da-wei;
[Objective] This paper aims to analyze response characteristics of desert grassland vegetation to climate change. [Method] The responses of grassland vegetation in desert region to air temperature/precipitation changes were analyzed by phenological observations since 1974, observation data concerning vegetation samples and meteorological data during the same period. The changing trend significance was denoted by linear trend line and its regression significance. The interrelation between the two variables was indicated by correlation coefficients. Data were analyzed by the software SPSS 13.0. [Result] (1) The rising rate of annual average temperature in the Minqin desert area since 1961 was greater than both the global level and China's level over the past century. (2) Desert plants' response to temperature changes were mainly displayed as the advance of spring phenology, the delay of autumn phenology and the extension of growing duration. Plants' main response to precipitation changes was that vegetation coverage and pure coverage were decreasing along with precipitation reducing. (3) Both the vegetation coverage and pure coverage mainly had a positive correlation with the annual precipitation. Plant density mostly had a positive correlation with the precipitation in September. (4) The impact of rising temperature on plant phenology in spring was greater than that in autumn. [Conclusion] The effects of temperature changes on desert grassland plants were the advance of spring phenology, the delay of autumn phenology, the extension of growing season and longer plant growing period than that in other reports. The responses of desert grassland vegetation to precipitation changes were that both vegetation coverage and pure coverage were decreasing along with precipitation reducing, and plant density fluctuated along with precipitation change.
2013年01期 v.5 41-49页 [查看摘要][在线阅读][下载 611K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：67 ] - YANG Ning;HUANG Hai;ZHANG Xi;WEI Yun;HU Hong-ming;WU Huo-lian;
[Objective] The aim was to summarize the artificial reproduction and fry rearing technique of Cichlasoma managuense. [Method] The artificial reproduction and fry rearing technique of Cichlasoma managuense were carried out. [Result] Two years old parent fish which cultured in nest can pair and spawn by themselves. The absolute fecundity of female fish were 1 500-2 500 eggs/fish, the relative fecundity were 500-800 eggs/fish. Fertilized eggs were elliptic, dark yellow, opaque, viscous, and arranged dispersedly in the nest. 50 of 72 pairs of parent fish were matched and spawned. The spawn rate was 74.2% and the fertility rate was 92.6% during the trial period. The time from fertilized egg to larva was 48 h± 41 min, Total body length of larva was 0.65±0.35 cm, hatching rate was 84.8%, total initial hatched fry was 401 000 in the water temperature 27-30 ℃. [Conclusion] 322 000 fishes of 3.0-5.0 cm fry were obtained in the cement pool with small cages cultivation in 41-43 d, survival rate 78.5%.
2013年01期 v.5 50-52页 [查看摘要][在线阅读][下载 106K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：91 ] -
<正>1. Requirement for Articles The journal mainly publishes original research papers, research notes, letters and reports about new technology on animal husbandry, veterinary medicine, feed science and other related fields. These articles can refer to basic theory, applied research and high-tech, etc. A manuscript must offer detailed document and reliable data. The innovative
2013年01期 v.5 53页 [查看摘要][在线阅读][下载 320K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：76 ] <正>Animal Husbandry and Feed Science is published to introduce the research achievements of animal husbandry and veterinary workers to the world, enhance their chances to participate in international academic exchange, and promote development of animal husbandry and veterinary. In 2009, Wu Chu (USA-China) Science & Culture Media Co. (Cranston, USA) and Anhui Wuchu Science, Technology
2013年01期 v.5 54页 [查看摘要][在线阅读][下载 263K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：60 ]