• Ultrastructure Observation of Cysticercus Pisiformis and Its Function Analysis

    PAN Yao-qian;ZHAO Zhen-sheng;CHEN Jin-shan;YIN Mei;TANG Hai-rong;LI Rui-zheng;LI Hui-zheng;LIU Zhi-ke;ZHANG Li-jie;LIU Xing-you;

    [Objective]The aim of the study was to observe the ultrastructure of Cysticercus pisiformis under different state. [Method] The Cysticercus pisiformis were obtained in the procedure of slaughter inspection form meat-packing plant. The Cysticercus pisiformis was taken out by cutting cyst directly, or cultured with physiologic saline containing 10% bile until evagination. Then it was observed and recorded by scanning electron microscopy. [Result]Observed from top of scolex of Cysticercus pisiformis locate in cyst, the rostellum looked like an umbrella. The cutaneous muscular columns with hamula covered the front end of scolex. Viewed from the side, a row of antlers-liking hamula adhered to the scolex. Four suckers were looked like more width and body segments less fime, in which there were lots of wrinkles in their surface respectively. The Cysticercus pisiformis cultured was divided obviously into scolex, neck, body segment and zone of cyst. When cutaneous muscular column of rostellum contracted, the antler-liking hamula extended to periphery that the ring-liking contraction appeared in neck and the lengthways contraction in body segments. [Conclusion] The Cysticercus pisiformis under different state has distinctly dissimilar ultrastructure.

    2012年04期 v.4 145-149+160页 [查看摘要][在线阅读][下载 1368K]
    [下载次数:22 ] |[网刊下载次数:0 ] |[引用频次:4 ] |[阅读次数:13 ]
  • Rapid Estimation on the Sperm Concentration in Boar Semen by Spectrophotometric Method

    PENG Yi;ZHANG Liang;

    [Objective] To establish the standardized spectrophotometric method to determine boar sperm concentration. [Method] The relationships between absorbance (A), transmittance (T) and sperm concentration (C) of different wavelengths (450, 550, 650 nm) were compared. [Result] The maximum sperm concentration detected by absorbance presented an upward trend with the increase of the wavelengths, 202 million/ml (450 nm), 224 million/ml (550 nm) and 235 million/ml (650 nm), respectively, but the stability of repeated measurement was decreased. With the increase of sperm dilution times, the stability of repeated measurement of transmittance was reduced, and when dilution times were more than 10 times (450 nm), 6 times (550 nm) and 4 times (650 nm), differences appeared between the observed values of repeated measurement. [Conclusion] Wavelength at 450 nm was found to be the most sensitive and reliable, and sperm concentration presented cubic functional regression relationship or power functional regression relationship with absorbance or transmittance, respectively. The regression equation for the standard curve at 450 nm was C450=0.48A3-0.76A2+0.67A-0.066 (R=0.951) and C450=1.657T-0.106 8 (R=0.940).

    2012年04期 v.4 150-152+168页 [查看摘要][在线阅读][下载 353K]
    [下载次数:16 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:18 ]
  • Effects of L-carnitine on Blood Lipid Metabolism and Antioxidation in Hyperlipidemia Rats

    LUAN Hai-yun;YANG Ming;XU Yong;WANG Gui-hua;

    [Objective] To study the effects of L-carnitine (LC) on lowering the high blood lipid and antioxidation in hyperlipidemia rats. [Method] 50 healthy SD rats were randomly divided into 5 groups. They were fed with standard diet, high-cholesterol diet and high-cholesterol diet with 0.25, 0.5, 1.0 g/(kg·d) LC. After the LC groups were consecutively orally administered LC for 28 days, rat serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C) content, as well as super oxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum and liver were determined. [Result] Compared with the high-fat model group, LC could significantly reduce the serum TC, TG, LDL-C levels, increase HDL-C level, and enhance SOD activity in serum and liver, decrease the content of MDA (P<0.05). [Conclusion] LC might have a significant role in lowering the high blood lipid and improving internal antioxidant capacity in hyperlipidemia rats.

    2012年04期 v.4 153-154+181页 [查看摘要][在线阅读][下载 87K]
    [下载次数:25 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:19 ]
  • Effects of Chinese Medicine on the Number of Intraepithelial Lymphocytes and Goblet Cells of Intestinal Villus of Heat Stress Layers

    GAO Gui-sheng;GAO Guang-ping;SHI Qiu-mei;ZHANG Yan-ying;SHEN Ping;SHAO Xin-hua;LIANG Yin-ju;PAN Fang-fang;

    [Objective] To study the effects of Chinese herbal additives on the number of intraepithelial lymphocyte and goblet cells of intestinal villus of heat stress layers. [Method] 180 healthy 88-day-old ISA brown egg roosters were selected and randomly divided into nine treatment groups, normal temperature control group, high temperature control group, VC group, prescription one high-dose group, prescription one middle-dose group, prescription one low-dose group, prescription two high-dose group, prescription two middle-dose group, prescription two low-dose group, respectively. Prescription one and two groups were respectively fed with low, medium and high concentrations of the three doses of Chinese herbal extracts, and VC group was fed with the VC in aqueous solution. Histological sections conventional technology and HE staining method were used to observe the number of intraepithelial lymphocytes and goblet cells in each sections of small intestine of chicken. [Result] The number of chicken intestinal epithelial lymphocytes and goblet cells showed a gradually decreasing trend in a high-temperature state. [Conclusion] Prescription one and two groups could promote the cytopoiesis of goblet cells and lymphocytes, and the effect of prescription two was the best. Moreover, Adding the Chinese herbs had good effects on relieving the heat stress of layers.

    2012年04期 v.4 155-160页 [查看摘要][在线阅读][下载 202K]
    [下载次数:26 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:22 ]
  • Effects of Pak Ning Powder and Oral Liquid Treating Chicken Infectious Laryngotracheitis

    GAO Gui-sheng;GAO Guang-ping;SHI Qiu-mei;ZHANG Yan-ying;SHEN Ping;SHAO Xin-hua;LIANG Yin-ju;

    [Objective] To investigate the therapeutic effects of Chinese herbal medicine compound preparation "Pak Ning" on chicken infectious laryngotracheitis. [Method] "Pak Ning" with different dosage forms and dosage time were used to carry out clinical observation of artificial infectious and natural infectious ILT of chickens. [Result] The therapeutic efficacy of "Pak Ning" oral liquid water was better than powder with high cure rate, the difference was significant (P<0.05). compared with Ribavirin, the difference was significant (P<0.01), and the preventive effects of feeding in advance were very well. [Conclusion] "Pak Ning" plays an important role in the treatment and prevention of ILT with strong infectiosity and high mortality.

    2012年04期 v.4 161-165页 [查看摘要][在线阅读][下载 369K]
    [下载次数:34 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:21 ]
  • The Effect of Traditional Chinese Medicine on Serum AMS,LPS and XOD of Piglets with Spleen Deficiency

    GAO Guang-ping;GAO Gui-sheng;ZHANG Dong-lin;ZHANG Yan-ying;SHI Qiu-mei;LIANG Yin-ju;SHAO Xin-hua;

    [Objective] The experiment aimed to determine the effect of Guchangcuzhangsan on serum amylase, lipase and xanthine oxidase of piglets with spleen deficiency. [Method] The model of piglets with spleen deficiency was produced by the methods of injecting reserpine into piglets, and alternating between no feed and good feed. The determinations of serum amylase, lipase and xanthine oxidase of piglets with spleen deficiency were done before injecting reserpine, on the day when reserpine was injected last time, the next day of finishing giving powder, the eighth day after finishing giving powder. [Result] After the experiment was finished, all 3 enzymes activities of Guchangcuzhangsan group had no significant difference from that of normal contrast group, enzyme activities of spleen deficiency group and smecta group had significant difference compared with that of normal contrast group.[Conclusion] Guchangcuzhangsan can promote the recovery of serum amylase, lipase and xanthine oxidase of piglets with spleen deficiency which was caused by reserpine.

    2012年04期 v.4 166-168页 [查看摘要][在线阅读][下载 91K]
    [下载次数:20 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:27 ]
  • The Detection of Pathogenic Porcine E.coli Virulence Gene (astA,stb)

    ZHANG Yan-ying;GAO Gui-sheng;GE Mu-xiang;GAO Guang-ping;SHI Qiu-mei;WANG Yan-yan;LIU Li-li;

    [Objective] This paper aimed to find out the relationship between pathogenic procine E.coli virulence gene and pathogenicity, and explore the pathogenic mechanism of E.coli. [Method] The detection of two E.coli virulence genes was performed. PCR method was taken to test the virulence genes, astA and stb, from 39 strains of typical serotype O porcine E.coli which had been separated and identified. [Result] It's found that of the 39 isolates of porcine E.coli, 22 carried virulence gene astA which represented 56.41%, and 27 carried virulence gene stb which represented 69.23%.[Conclusion] This study has provided scientific data for future E.coli pathogenicity researches.

    2012年04期 v.4 169-171+175页 [查看摘要][在线阅读][下载 144K]
    [下载次数:33 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:25 ]
  • Study on Genetic Inactivation Bacterial Ghosts of Pasteurella multocida based PhiX174 Gene E Lysis Cassette Mediated

    ZHU Bi-feng;YANG Xu-fu;LIU Dong-sheng;

    [Objective] To study on genetic inactivation bacterial ghosts of Pasteurella multocida based PhiX174 gene E lysis cassette mediated. [Method] Recombinant pPBA1100-e was constructed by which the gene E of bacteriophage Phix174 and temperature sensitivity expressing control system hybridized with plasmid pPBA1100 by genetic engineering method. Recombinant was transformed to Pasteurella multocida and lysis gene E expressed by temperature induction. Recombinant was detected by restriction endonuclease. Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysis. [Result] The results indicated that the recombination plasmid presented three bands by restriction endonuclease and agarose electrophoresis and that molecular weight of every band accorded with theoretical value. The result of SEM observing showed that recombination plasmid expressed successfully in P. multocida and produced bacterial ghost. The result of CFU detecting demonstrated that inactivation ratio of P. multocida reached 99 per cent. [Conclusion] This study provided technical bases for the preparation of antigen vaccine of natural bacterial outer membrane protein.

    2012年04期 v.4 172-175页 [查看摘要][在线阅读][下载 278K]
    [下载次数:41 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:16 ]
  • Research on 30 days Feeding Test of Rats with Irradiated Pet Food

    ZHU Jia-ting;YAN Jian-min;FENG Min;LI Li;YANG Ping;WANG De-ning;GU Gui-qiang;

    [Object] The research is aimed to provide scientific basis for irradiated pet food's standardized production, supervision and commercialized application. [Method] Take broiler breast meat with high-dose irradiation as testing material, then evaluate its safety after 30 days feeding test of rats [Result] Compared with control group (Pet food's irradiation dose is 0 kGy), the general situation, weight, food-intake, food utilization rate, hematological examinations, bio-chemical analysis of blood, viscera weight, ratio of viscera and body weight, histopathological examinations, physiological feature, life's basic characteristics (including height, tail length, body temperature, heart rate and blood pressure ) and other indexes of rats, which were fed respectively with 10, 15, 25 kGy irradiation dose pet food for 30 days, were not found abnormal. [Conclusion] Pet food with 10, 15, 25kGy irradiation dose is safe.

    2012年04期 v.4 176-181页 [查看摘要][在线阅读][下载 129K]
    [下载次数:30 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:26 ]
  • Effects of Coated Methionine on the Growth Performance, Blood Biochemical Indices and Nutrient Apparent Ileal Digestibility in Broilers

    ZHANG Yong;HAN Chun-xiao;ZHU Yu-jing;JI Wen-yan;SHAO Cai-mei;

    [Objective] This trial was conducted to study the effects of different coated methionines on the growth performance, blood biochemical indices and apparent ileal digestibility of amino acids in broilers. [Method] A total of 3 780 Cobb 500 broilers aged 18 days were randomly arranged into 7 groups with 3 replicates per group and 180 broilers per replicate. Control group (A) received the basal diet supplemented with 0.16% DL-methionine, and trial groups received the basal diets supplemented with 0.32% (B), 0.29% (C), 0.26% (D), 0.22% (E), 0.19% (F) and 0.16% (G) of coated methionine, and broilers were fed to the age of 42 days. [Result] The final weight, average daily gain and average daily feed intake of groups B and C were higher than those of the control group (P<0.05), and the mortality of group B was lower than that of the control group (P<0.05), but there was no significant difference in all above indices between the other groups and the control group (P>0.05). Plasma GSH-Px activity of trial groups (except group G) was higher than that of the control group (P<0.05), and SOD activity of groups B and C were higher than that of group G (P<0.05), but there were no significant differences in other blood biochemical indices among all groups (P>0.05). There were no significant differences in apparent ileal digestibility of dry matter and crude protein between trial groups and the control group (P>0.05), and the apparent ileal digestibility of amino acids of trial groups was increased (P<0.05) or no change expect that the apparent ileal digestibility of many kinds of amino acids of group G was decreased compared with the control group (P<0.05). [Conclusion] The growth performance and apparent ileal digestibility of amino acids in broilers were increased with the rising supplementation of coated methionine, but the supplementation of 0.19% coated methionine can meet the needs of broilers, namely, coated methionine can be substituted for 60% DL-methionine in the diet, so DL-methionine can be saved up to 40%.

    2012年04期 v.4 182-186+192页 [查看摘要][在线阅读][下载 155K]
    [下载次数:21 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:19 ]
  • Effect of K~+ and the Interaction of Major Ions on Survival,Growth and ATP Enzyme Activity of Litopenaeus vannamei

    ZHANG Li-tian;DAI Xi-lin;ZHANG Dong;

    [Objective] On the basis of previous researches, this experiment aimed to analyze the effect of K+ and the interaction of K+, salinity(s), Ca2+, Mg2+ on growth and adenosine triphosphatase (ATPase), which was closely related to growth, of Litopenaeus vannamei. [Method] 5-week Litopenaeus vannamei breeding experiment was performed according to L16(215) orthogonal experiment design. Then the effect of K+ and the interaction of K+, salinity, Ca2+, Mg2+ in water on survival, growth and ATP enzyme activity of Litopenaeus vannamei. [Result] K+ had significant effect on survival rate, length and weight gain, ATP enzyme activity in Litopenaeus vannamei. The highest survival rate was at K+ of 150 mg/L. The fastest growth rate was at 50 mg/L, as well as the activities of Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase. The interaction of salinity, Ca2+, Mg2+, K+ in water also had significant effect on survival rate, length and weight gain, ATP enzyme activity in Litopenaeus vannamei. [Conclusion] Among the horizontal combinations of factors in the experiment, the optimal combination for Litopenaeus vannamei survival rate was salinity of 15, K+ of 150 mg/L, Ca2+ of 100 mg/L, Mg2+ of 300 mg/L, the optimal combination for growth rate was salinity of 15, K+ of 50 mg/L, Ca2+100 mg/L, Mg2+100 mg/L, the optimal combination for Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase activity in Litopenaeus vannamei was salinity of 5, K+ of 50 mg/L, Ca2+ of 100 mg/L, Mg2+ of 100 mg/L.

    2012年04期 v.4 187-192页 [查看摘要][在线阅读][下载 156K]
    [下载次数:12 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:17 ]
  • About Animal Husbandry and Feed Science

    <正>Animal Husbandry and Feed Science is published to introduce the research achievements of animal husbandry and vet-erinary workers to the world, enhance their chances to participate in international academic exchange, and promote development of animal husbandry and veterinary. In 2009, Wu Chu (USA-China) Science & Culture Media Co. (Cranston, USA) and Anhui Wuchu Science, Technology and Culture Communication Co., Ltd (Hefei, China) issued the journal Animal Husbandry and Feed Science (ISSN 1943-9911). The main content is basic theory a

    2012年04期 v.4 194页 [查看摘要][在线阅读][下载 246K]
    [下载次数:5 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:17 ]
  • Instructions for Authors

    <正>1.Requirement for Articles The journal mainly publishes original research papers, research notes, letters and reports about new technology on animal husbandry, veterinary medicine, feed science and other related fields. These articles can refer to basic theory, applied research and high-tech, etc. A manuscript must offer detailed document and reliable data. The innovative idea should be described concisely and definitely. A review should report the recent progress in animal science, and it should be

    2012年04期 v.4 193页 [查看摘要][在线阅读][下载 310K]
    [下载次数:4 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:24 ]
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