• Comparative Advantages and Specialized Production of Beef Cattle Industry in Yanbian Region

    [Objective] To provide theoretical guidance for development of beef cattle industry in Yanbian region. [Method] Aggregated comparative advantage index and concentration factor were used to compare beef industry in Yanbian region and Jilin Province. [Result] Beef cattle industry in Yanbian region had aggregated comparative advantages, and its concentration factor tended to rise year by year. [Conclusion] The beef cattle industry in Yanbian region has obvious comparative advantages and development potential.

    2011年03期 v.3 1-3页 [查看摘要][在线阅读][下载 90K]
    [下载次数:39 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:12 ]
  • Effects of Non-starch Polysaccharide Enzyme Products on Meat Performance in Broilers

    [Objective] To investigate the effects of non-starch polysaccharide (NSP) enzyme products on meat performance of broilers and thus to provide a scientific basis for their rational use. [Method] Two hundred 21-day-old Ross broilers were assigned to five groups. The broilers in group I and group II were fed positive control diet and negative control diet, respectively, which were slightly different. The broilers in group III, IV and V were fed the negative control diet respectively supplemented with different commercial NSP enzyme products. The broilers were slaughtered at 56 days old, and their meat yield was determined. [Result] The percentage of carcass yield and percentage of abdominal fat were significantly higher in the group III than in the group II (P < 0.05). The percentage of small intestine weight in slaughter weight was lower in the group II than in other groups. [Conclusion] Diets supplemented broad-rang and highly active NSP enzyme products can promote absorption of routine feed ingredients, increase meat yield, and improve meat performance.

    2011年03期 v.3 4-6页 [查看摘要][在线阅读][下载 82K]
    [下载次数:22 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:10 ]
  • Production Technology and Application of Slow-Release Non-protein Nitrogen Extruded Feed Additive

    Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release non-protein nitrogen extruded feed. By designing the best formula and using chelating and emulsifying process, the slow-release non-protein nitrogen extruded feed additives were produced. This product increases milk yield and improves milk quality, thus increasing economic efficiency.

    2011年03期 v.3 7-8+12页 [查看摘要][在线阅读][下载 99K]
    [下载次数:21 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:9 ]
  • Effect of Corn Stalks Treated with Carbonylamines in Different Concentrate and Forage Rations and Nitrogen Conditions on Rumen Fermentation in Vitro

    [Objective] To investigate the effects of concentrate/roughage ratios and nitrogen levels on in-vitro rumen fermentation of urea-treating corn stalk. [Method] The concentrate/roughage ratios were 7∶3, 5∶5 and 3∶7. The crude protein levels were 11% and 14%. The in-vitro culture time was 2, 4, 6 and 48 h. [Result] The pH value of broth decreased significantly with the decline in the proportion of roughage (P < 0.01) and with the increase in the crude protein levels (P < 0.05). The ammonium nitrogen concentration and acetic acid/propionic acid ratio of the fermented products decreased with the increase in the crude protein levels and proportion of concentrate in diet. The diet with concentrate/roughage ratio of 5∶5 and crude protein level of 14% had significantly higher digestibility of dry matter and organic matter than other diets (P < 0.05). The digestibility of organic matter increased gradually with the increasing proportion of concentrate and crude protein level. With the increase in the proportion of concentrate, the microbial protein levels increased remarkably, while the acetic acid/propionic acid ratio declined. [Conclusion] The concentrate/roughage ratios and nitrogen levels affect rumen fermentation and microbial growth during in-vitro culture. However, the best supplementary feeding results of urea-treating corn stalks can be obtained when the concentrate/roughage ratio is below 5∶5 and the crude protein level is 14%.

    2011年03期 v.3 9-12页 [查看摘要][在线阅读][下载 193K]
    [下载次数:31 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:9 ]
  • Sub-chronic Toxicity Test of Tephrosia canadida DC. in Mice

    [Objective] To observe sub-chronic toxicity of Tephrosia canadida DC. in mice and thus to evaluate their safety to be used as protein feed resources. [Method] Sixty-four 6-week-old healthy Kunming mice weighing about 25 g were randomly divided into four groups (n = 16), half male and half female. Mass ratios of basic diet to leaf meal of Tephrosia canadida DC. in group A, group B, group C and group D were 10∶ 0, 4∶ 1, 3∶ 2 and 2∶ 3, respectively. After 35-d feeding, the effects of Tephrosia canadida DC. on growth, blood and organs of mice were observed. [Result] During the trial, all mice had normal activities, and no death and no abnormal blood index were observed. All organs of the mice in the experimental groups had no visible pathological lesion, and organ indexes had no significant difference between the experimental groups and the control group. Except that slightly abnormal histological changes appeared in liver and kidney of the mice in the group C and D, no histological change was observed in other organs of the experimental mice. [Conclusion] Tephrosia canadida DC. have no adverse effects on mice, which provides a reference for research about their safety in feed of other animals.

    2011年03期 v.3 13-15+19页 [查看摘要][在线阅读][下载 263K]
    [下载次数:22 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:8 ]
  • Determination of Physiological and Biochemical Indicators of Brown-Egg Dwarf Layers at Different Ages

    [Objective] To observe biological characteristics of brown-egg dwarf layers. [Method] Physiological and biochemical indicators of brown-egg dwarf layers were determined at the age of 20, 40 and 60 weeks, respectively. Fifty-four chickens were detected, nine roosters and nine hens at each age. [Result] As analyzed by t test and multiple comparisons, during 20-60 weeks old, the RBC, HGB, MPV, PDW, LYMPH%, TP and CHO showed an increasing trend. However, during this period, the HCT, MCV, RDW, WBC and LYMPH first decreased and then declined, while the MCH, MCHC, PLT, PCT, MID, MID%, GRAN, GRAN%, AST, ALB, TG, ALT and GLU changed contrarily. The chickens at different ages had significantly different physiological and biochemical indicators (P < 0.05). The RBC, HCT, HGB, WBC, LYMPH and AST were significantly different between the roosters and hens during 20-60 weeks old. [Conclusion] In the brown-egg dwarf layers, the physiological and biochemical indicators vary with aging, and their difference levels also change with aging.

    2011年03期 v.3 16-19页 [查看摘要][在线阅读][下载 99K]
    [下载次数:24 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:9 ]
  • Effect of Follicle-Stimulating Hormone Dosage, Seasons and Treatment Frequency on Super-Ovulation in Goats

    [Objective] To explore the effects of FSH (Follicle-stimulating hormone), seasons and treatment frequency on super-ovulation in donor goats and to provide necessary data for research about embryo transfer and embryo biotechnology in Yangzhou area. [Method] Two FSH dosages (240 and 300 IU), two seasons (April-June and October-December), and two treatment frequencies (one or two times) were used to induce super-ovulation in goats. [Result] Both average ovulation point and number of transferable embryos were significantly different between the goats given 240 IU FSH and those given 300 IU FSH (average ovulation point, 10.12 vs 15.55; number of transferable embryos, 8.82 vs 13.15) at the 0.05 level. Both average ovulation point and number of transferable embryos were also significantly different between April-June and October-December (average ovulation point, 9.05 vs 15.55; number of transferable embryos, 7.05 vs 13.15) at the 0.05 level. Super-ovulation effect was not significantly different between the two treatment frequencies. [Conclusion] The FSH dosages and seasons have significant impact on super-ovulation, but repeat super-ovulation does not have the same impact.

    2011年03期 v.3 20-22页 [查看摘要][在线阅读][下载 89K]
    [下载次数:16 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:12 ]
  • Progress in Determination Methods of Degradation Rate in Ruminants

    Degradation rate of feed proteins in rumen is a basic indicator of new intestinal protein system of ruminants. In this paper, determination methods of degradation rate in rumen including in-vivo method, nylon bag method and artificial rumen method are compared in order to provide a reference for animal nutrition.

    2011年03期 v.3 23-24+28页 [查看摘要][在线阅读][下载 98K]
    [下载次数:12 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:5 ]
  • Recent Advances in Epidemiology of Porcine Circovirus Type 2 and Diagnosis of Porcine Circovirus-Associated Diseases

    Porcine circovirus (PCV) is the smallest animal virus so far and has two serotypes. PCV1 is nonpathogenic, but PCV2 is pathogenic and causes post-weaning multisystemic wasting syndrome (PMWS). Factors to induce PMWS include immunity and infection status of sows, infection time, mixed infection, PCV2 variants, physical status of gilts, and feeding management. For final diagnosis, histopathological changes and existence of PCV2 in lymphoid tissues are professional standards, because fluorescence quantitative RT-PCR is not enough specific or sensitive. The commercial PCV2 vaccines can reduce occurrence of PMWS and PCV-related diseases. This paper reviews recent advances in epidemiology of PCV2 as well as diagnosis and control of PMWS.

    2011年03期 v.3 25-28页 [查看摘要][在线阅读][下载 109K]
    [下载次数:42 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Development of an Efficient Method for Extracting Total DNA of Intestinal Microflora

    [Objective] The aim was to develop a fast and effective DNA extraction method of intestinal microflora, a modified method of chloroform extraction, and to provide the basis for quantitative and qualitative detection. [Method] Through the improvement of conventional DNA extraction method, a rapid and efficient DNA extraction method was developed. Compared with the real-time PCR result of control sample and the result of QIAamp DNA Stool Mini kit, the developed method was verified. [Result] The DNA yield of the developed method was 100 times as much as that of QIAamp DNA Stool Mini kit. And the real-time PCR result showed that the efficiency of DNA extraction of the developed method was higher than that of the QIAamp DNA Stool Mini kit. [Conclusion] This modified method is inexpensive, efficient and rapid, and it is suitable for large quantities of feces samples.

    2011年03期 v.3 29-31页 [查看摘要][在线阅读][下载 207K]
    [下载次数:39 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Research Progress in NTPase of Toxoplasma gondii and Other Protozoa

    Nucleoside triphosphate hydrolase (NTPase) is a multifunctional enzymatic family widely existing in vivo. They can hydrolyze NTP to NMP or dNTP to dNMP to produce energy. In this article, the structure of Toxoplasma gondii NTPase is analyzed. The research progress in NTPase of Toxoplasma gondii, Trypanosoma cruzi, Sarcocystis neurona and Neospora caninum was briefly reviewed.

    2011年03期 v.3 32-34页 [查看摘要][在线阅读][下载 95K]
    [下载次数:18 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:11 ]
  • Development of an Indirect Sandwich ELISA for Detection of Duck Swollen Head Septicemia Virus

    [Objective] To develop an indirect sandwich ELISA for rapid detection of duck swollen head septicemia virus (DSHSV). [Method] DSHSV hyperimmune sera were prepared in ducks and rabbits by inoculation with DSHSV XD strain and then purified using saturated ammonium sulfate and Sephadex G-150 column chromatography to obtain anti-DSHSV IgG. An indirect sandwich ELISA was developed using the purified duck anti-DSHSV IgG and rabbit anti-DSHSV IgG after reaction conditions were optimized. Its specificity, sensitivity and repeatability were evaluated, and its accuracy was confirmed by observation with immunoelectron microscopy. Then, distribution of DSHSV in tissues of challenged ducks was also detected. [Result] Through optimizing conditions, the ELISA was developed. Only DSHSV could be detected by the developed method, but other pathogens could not be detected. Compared with agar gel diffusion test, the developed method was more sensitive. The coefficient of variation was less than 10%, and the developed method had good repeatability. In addition, the ELISA-positive samples contained DSHSV, as confirmed by immunoelectron microscopy. All heart, liver, lung and kidney collected from the DSHSV-challenged ducks were positive when they were detected by the developed ELISA. [Conclusion] The developed ELISA method is rapid, simple, specific and sensitive, and it is suitable for large-scale quarantine of DSHS. Heart, liver, lung and kidney should be selected preferentially as specimens for diagnosis of DSHS.

    2011年03期 v.3 35-37+44页 [查看摘要][在线阅读][下载 114K]
    [下载次数:15 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Preparation of Sporozoite Antigen of E. tenella

    [Objective] To provide antigen for preparation of monoclonal antibodies against E. tenella. [Method] Broiler chickens were inoculated with axenic culture of sporulated oocysts of E. tenella, and their feces were collected after 5-10 d. After simple separation, the oocysts were sporulated, purified and counted to prepare sporozoite antigen. [Result] This method used to prepare sporozoite antigen was simpler and easier than other methods. In addition, it required few specialized equipment and media, and it could be conducted in general laboratories. However, it needed long time. [Conclusion] The E. tenella oocysts have been isolated from chickens, and the sporozoite antigen with high protein concentration has been obtained.

    2011年03期 v.3 38-39+48页 [查看摘要][在线阅读][下载 135K]
    [下载次数:10 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:10 ]
  • Application of Highly Efficient Microbial Agent in Pig Manure Compost

    [Objective] The aim of this study was to develop a cheap and localized microbial agent so as to solve high cost of microbial agent for pig manure composting in Jiaxing City. [Method] Pig manure in the experimental group and control group was inoculated with the self-developed microbial agent and commercial microbial agent, respectively. The manure was decomposed for 38 d, during which the indicators of compost including physical properties, temperature, pH value, water content, organic matter, dissolved nitrogen, carbon nitrogen ratio and germination rate were studied. [Result] The water content in the experimental group declined to 26.10% after 33 d of compost, meeting the standard upper limitation of 30% for maturity. By comparison, the water content in the control group was slightly higher than 30% even after 38 d. The germination rate of seeds fertilized with the experimental manure compost met the standard for maturity on Day 28, while that in the control group met the standard for maturity on Day 35. When the composting was finished, the ratio of total carbon to total nitrogen in the pig manure was 14.64 and 16.43 respectively in the experimental and control group, and the organic matter content was about 45% for both. All these indexes could meet the standards for organic fertilizer products. [Conclusion] The self-developed microbial agent can meet the requirements for pig manure composting, and it can shorten the composting time by 5-8 d compared with the commercial agent. In addition, the fertilizer product composted by the self-developed microbial agent has lower water content and thereby is much more beneficial for preservation.

    2011年03期 v.3 40-44页 [查看摘要][在线阅读][下载 399K]
    [下载次数:66 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:43 ]
  • Teaching Form Reform of "Prevention and Control of Poultry and Poultry Diseases" Course in Higher Vocational Colleges

    [Objective] To meet the needs of "Prevention and Control of Poultry and Poultry Diseases" course in higher vocational colleges in new period. [Method] In order to introduce theoretical knowledge, enhance practical ability and train quality, teaching content, teaching methods and practical teaching of the course were explored according to trends of academic development and personnel training. [Result] The reform of teaching form improved the teaching effect, mobilized the learning enthusiasm and initiative of students, and enhanced their ability to solve practical problems. [Conclusion] The teaching form of "Prevention and Control of Poultry and Poultry Diseases" course has been successfully reformed.

    2011年03期 v.3 45-48页 [查看摘要][在线阅读][下载 226K]
    [下载次数:32 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:5 ]
  • Instructions for Authors

    <正>1. Requirement for Articles The journal mainly publishes original research papers,research notes, letters and reports about new technology on animal husbandry, veterinary medicine,feed science and other related fields. These artcles can refer to basic theory,

    2011年03期 v.3 49页 [查看摘要][在线阅读][下载 274K]
    [下载次数:3 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:5 ]
  • About Animal Husbandry and Feed Science

    <正>Animal Husbandry and Feed Science is published to introduce the research achievements of animal husbandry and veterinary workers to the world, enhance their chances to participate in international academic exchange, and promote

    2011年03期 v.3 50页 [查看摘要][在线阅读][下载 226K]
    [下载次数:11 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:7 ]
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