• Progress in Sex Identification of Early Embryo of Livestock

    Principle, research, and problems of techniques for sex identification of early embryo of livestock were discussed in detail from aspects of main ways for sex control, main methods for sex identification of early embryo, and application of sex identification in livestock production. And development prospects were expected.

    2010年01期 v.2 1-3+10页 [查看摘要][在线阅读][下载 240K]
    [下载次数:42 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:13 ]
  • Meat Production Performances of Geese in Different Crossbred Combinations

    [Objective] To study the meat production performance of geese in different crossbred combination, and provide a reference for cultivating new multiplication strains of meat geese. [Method] A total of five goose breeds including Taihu goose (T), Wulong goose (WL), Wanxi white goose (WX), Sichuan white goose (SC) and Sanhua goose (SH) were used as research objects, and eight crossbred combinations were designed. The body measurements, slaughter performance, conventional meat quality and muscle composition of 90-day-old geese in eight crossbred combinations were determined and comparatively analyzed. [Result] All the body measurement indexes except thurl width of WXSC combination were the biggest in the eight crossbred combinations. Contrarily, the body measurement indexes of SHSC combination, including average body weight, body slope length, breast depth, breast width, shank length, neck length and half-diving depth were the lowest in the eight crossbred combinations. The live weight, slaughter weight and abdomen fat weight of WXSC combinations ware 3 291.36, 2 956.46 and 77.84 g, respectively, which were the biggest in eight crossbred combinations. The slaughter rate of SHWL combinations was 92.96%, and more than other crossbred combinations. The shear force of SHT and WXWL combinations were the lowest (3.09 kg) and the highest (3.78 kg), respectively. The water holding capacities of SCWL and WXSC combinations were the highest (66.46%) and the lowest (61.48%), respectively. The percentage of moisture content of SHWL and SCT combinations were the highest (73.05%) and the lowest (66.77%), respectively. The crude protein content of SHT and SHWL combinations were the highest (23.32%) and the lowest (21.94%), respectively. The percentage of crude fat content of SHT and SCWL combinations were the highest (3.06%) and the lowest (1.67%), respectively. [Conclusion] The growth rate of WXSC combination is the fastest, and the meat performance of SHT combination is the best.

    2010年01期 v.2 4-7页 [查看摘要][在线阅读][下载 129K]
    [下载次数:26 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:18 ]
  • Localization of Porcine Neturopetide Y-Y1 mRNA in Hypothalamus-Pituitary-Ovary Axis during Puberty

    [Objective] To observe the disposition and localization of neturopetide Y-Y1(NPY-Y1) mRNA in hypothalamus, pituitary and ovary during puberty. [Method] Three 60-day-old Sujiang sows weighing 20 kg and three 160-day-old Sujiang sows weighing 80 kg were selected and anaesthetized. The hypothalamus, pituitary and ovary were taken out for preparation of frozen sections. The expression and localization of the NPY-Y1 mRNA in hypothalamus-pituitary-ovary axis was observed by the in situ hybridization. The PBS was substituted for hybridization solution to set up a control. [Result] Positive hybridization signals of the NPY-Y1 mRNA were detected in the hypothalamus, pituitary and ovary at different developmental stages, and these signals were stronger in the 60-day-old sows (before the puberty) than in the 160-day-old sows (during the puberty). [Conclusion] The NPY-Y1 mRNA is distributed in the hypothalamus-pituitary-ovary axis and can regulate reproductive process of sows.

    2010年01期 v.2 8-10页 [查看摘要][在线阅读][下载 357K]
    [下载次数:6 ] |[网刊下载次数:0 ] |[引用频次:3 ] |[阅读次数:17 ]
  • Effects of Microbial Fermentation Feeds on Growth Performance of Weaned Piglets

    [Objective] To study the effects of microbial fermentation feeds on growth performance of piglets, and provide the basis for their promotion and application. [Method] All of 60 Duroc×Landrace×Yorkshire crossbred weaned piglets, weighing 8-10 kg, were randomly housed in six groups, 10 piglets in each group. The piglets in control groups were fed with traditional daily food which contained aureomycin at 70 mg/kg·BW and concentrate feeds without antibiotics. While the piglets in experimental groups were fed with concentrate feeds without antibiotics and microbial fermentation feeds. [Result] The results indicated that the average daily gain of experimental groups raised by 5.56%, and the feed conversion ratio reduced by 3.53% compared to control group. Moreover, the number of probiotics in the feces of experimental groups increased and pH value decreased significantly compared with the control groups (P<0.05). [Conclusion] Microbial fermentation feeds can be used to replace antibiotics feeds, and promote healthy growth of the piglets.

    2010年01期 v.2 11-13页 [查看摘要][在线阅读][下载 112K]
    [下载次数:91 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:57 ]
  • Phagocytosis Conditions of Peripheral Blood Leucocytes in Chicken

    [Objective] To investigate the optimal conditions of peripheral blood leucocytes phagocytosing Staphylococcus spp. [Method] With Staphylococcus spp. 10 145 as tested materials, phagocytosis activity of chicken′s peripheral blood leucocytes was detected by the method of Wright′s staining. The effects of time, temperature and concentration of Staphylococcus spp. 10 145 on phagocytosis percentages (PP) and phagocytosis index (PI) were optimized by orthogonal test and their morphologic characteristics were observed by microscope. [Result] The concentration of Staphylococcus spp. 10 145 was the primary factor effecting leucocytes phagocytosis index. The optimal phagocytosis conditions were at the temperature of 41 ℃, time of 40 min and concentration of Staphylococcus spp. 10 145 of 5×106/ml. The order of various factors on leucocytes phagocytosis index was the concentration of Staphylococcus spp. 10 145 > time > temperature. [Conclusion] This study provides a more simple and reliable method for detecting leucocytes phagocytosis function.

    2010年01期 v.2 14-16页 [查看摘要][在线阅读][下载 196K]
    [下载次数:7 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:15 ]
  • Effects of Garlic Oxide on Movement of Ruminal Smooth Muscle in Goats

    [Objective] To explore the effects of garlic oxide on movement of ruminal smooth muscle in goat and provide a theoretical basis for metabolic regulation of rumen. [Method] A total of four healthy adult goats were selected and respectively implanted a permanent rumen fistula by surgery. After the garlic oxide was poured into the rumen of goats, the movement of ruminal smooth muscle in vivo was observed by self-developed ruminal smooth muscle movement detector. [Result] The average muscle contraction intensity of the four goats was significantly increased by 51.6% (P<0.01) in experiment period compared with that of the control. The average contraction frequency was significantly increased by 24.6% (P<0.01). The average duration of contraction was significantly shortened by 19.1% (P<0.01). The average feeding intake was significantly increased by 8.1% (P<0.01). [Conclusion] Garlic oxide can greatly strengthen the movement of ruminal smooth muscle and promote gastric emptying.

    2010年01期 v.2 17-19+23页 [查看摘要][在线阅读][下载 350K]
    [下载次数:14 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:20 ]
  • Sequence Analysis on Interleukin-2 Gene and Interleukin-6 Gene in a Hybrid Pig

    [Objective] To understand the functions of interleukin-2 (IL-2) and interleukin-6 (IL-6) in immune response. [Method] Peripheral lymphocytes were isolated from a hybrid pig (Duroc×Landrace×Shandong local pig) and stimulated with canavalin A in vitro. With total RNA as templates, the IL-2 and IL-6 cDNA were amplified by the RT-PCR and sequenced, respectively. [Result] The sequence of IL-2 gene had 100.0% nucleotide homology to the published IL-2 sequences. The sequence of IL-6 gene had 99.8%-100.0% nucleotide homology to the published IL-6 sequences, and the amino acid homology was 99.1%-100.0%. [Conclusion] No great variation of the IL-2 gene and IL-6 gene is observed in the hybrid pig, and these results provide a basis for research about functions of IL-2 and IL-6 protein.

    2010年01期 v.2 20-23页 [查看摘要][在线阅读][下载 268K]
    [下载次数:10 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:13 ]
  • Effects of Diets with Different Corn-to-Soybean Ratios on Performance of Lactational Rex Rabbits

    [Objective] To study the effects of diets with different corn-to-soybean ratios on production performance of lactational Rex rabbits and select the best feed formula. [Method] A total of 80 lactational healthy Rex rabbits (similar age, body weight and delivery time) were randomly divided into five groups, 16 in each group. The Rex rabbits in group I were fed with corn-based meal diet, group II with soybean-based meal diet, group III with the developed corn-soybean meal diet, group IV with corn-soybean meal free diet, and group CK with conventional corn-soybean meal diet. During the lactation period, the changes in the lactation performance, growth rate of baby rabbits, weight and feed costs were observed. [Result] The lactation performance of mother rabbits and the growth rate of baby rabbits in the experimental groups were similar to that in the control group. The weaning survival rate of the group III was the highest, but it was not significantly higher than that of the control group. The weaning survival rate of the other three groups was lower than that of the control group (P<0.05). The weight loss of mother rabbits in the group I, II and III was not significantly different from that of the control group, but the weight loss of the group IV was higher than that of the control group (P<0.01). [Conclusion] Diets with appropriate corn-to-soybean ratios not only reduce feed costs but also give the best feeding effects.

    2010年01期 v.2 24-26+31页 [查看摘要][在线阅读][下载 122K]
    [下载次数:20 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:17 ]
  • Effects of Different Dietary Crude Protein Levels on Reproductive Performance in Breeder Roosters

    [Objective] To investigate effects of different dietary crude protein levels on reproductive performance and find out the optimal additive proportion of crude protein. [Method] A total of 45 New Yangzhou breeder roosters at 21 weeks old were sampled and divided into three groups, namely, low-protein diet group, medium-protein diet group and high-protein diet group. They were fed with diets respectively at crude protein levels of 17.23%, 13.04% and 11.32%. The sperm volume, testicular traits and levels of reproductive hormones were analyzed during the whole reproductive period. [Result] The sperm volume, sperm motility and sperm density of the medium-protein diet group were higher than that of the other two groups, and the sperm deformity rate of the medium-protein diet group was lowest. The rooster′s testicular traits including weight, volume and testis indexes were not significantly affected by different protein levels, but the testis shrank slowly with aging (P<0.05). The levels of testosterone (T), luteinizing hormone (LH) and follicule stimulating hormone (FSH) in plasma were respectively 6.34%, 19.64% and 3.88% higher in the low-protein diet group than in the medium-protein diet group, and these indexes were respectively 7.20%, 17.54% and 9.84% higher in the low-protein diet group than in the high-protein diet group. But all these differences were not significant. [Conclusion] The breeder roosters fed with the diet at the crude protein level of 13.04% show better reproductive performance and economic efficiency.

    2010年01期 v.2 27-31页 [查看摘要][在线阅读][下载 223K]
    [下载次数:18 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:10 ]
  • Investigation on Avian Leukosis Virus Subgroup A in Layers in Guizhou Province of China

    A total of 273 serum samples were collected from 10 layer breeding farms in Guizhou Province and detected by enzyme-linked immunosorbent assay. The positive rate of antibodies against avian leukosis virus reached 14.64%. Then the samples from cases of hemangioma in one of the 10 breeding farms were detected by polymerase chain reaction, and the diagnosis indicated that the hemangioma was caused by avian leukosis virus subgroup A.

    2010年01期 v.2 32-33+37页 [查看摘要][在线阅读][下载 203K]
    [下载次数:19 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:16 ]
  • Rapid and Visualized Detection Method of Canine Parvovirus Using Loop-Mediated Isothermal Amplification

    [Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification (LAMP), improve the detection rate of canine parvovirus (CPV) and reduce the testing cost. [Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [Result] The optimal reaction time of the LAMP method for CPV was 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.

    2010年01期 v.2 34-37页 [查看摘要][在线阅读][下载 235K]
    [下载次数:40 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:11 ]
  • Development of an Indirect ELISA Using Recombinant Truncated Envelope Glycoprotein for Detection of Antibodies against Japanese Encephalitis Virus

    [Objective] To develop an indirect ELISA assay for detecting antibodies against envelope glycoprotein (E protein) of Japanese encephalitis virus (JEV). [Method] Specific primers were designed according to JEV sequences published in the GenBank. The cDNA of JEV E gene (about 1 000 bp) was amplified by the RT-PCR with the specific primers. After sequencing analysis, the E gene was cloned into pET30a expression vector and expressed in E. coli BL21(DE3) with the induction of IPTG. After denaturation, purification and renaturation, the recombinant protein was analyzed by the SDS-PAGE and the western blotting. An indirect ELISA was developed to detect antibodies against JEV. [Result] The E protein was mainly expressed in inclusion body. With the purified E protein, the indirect ELISA was developed and displayed good specificity, sensitivity and repeatability. [Conclusion]The developed ELISA using the truncated E protein as antigen is a simple, convenient and rapid serological method for diagnosis, monitoring antibody level and epidemiological investigation of JEV.

    2010年01期 v.2 38-42页 [查看摘要][在线阅读][下载 333K]
    [下载次数:20 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:17 ]
  • Diagnosis and Treatment of Swine Toxoplasmosis

    Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii, which mainly affects pigs, cattle, sheep, rabbits and other animals. In addition, human beings are also susceptible to Toxoplasma gondii. Because Toxoplasma gondii infections are widely prevalent in human beings and other animals, we should pay more attention to prevention and control of the disease in order to promote the healthy development of breeding industry and the protection of people′s health. In the present article, the pathogen of swine toxoplasmosis, life history, clinical features and treatment are elaborated.

    2010年01期 v.2 43-48页 [查看摘要][在线阅读][下载 368K]
    [下载次数:19 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:16 ]
  • Instructions for Authors

    <正>1.Requirement for Articles The journal mainly publishes original research papers, research notes, letters and reports about new technology on animal husbandry, veterinary medicine, feed science and other related fields. These articles can

    2010年01期 v.2 49页 [查看摘要][在线阅读][下载 282K]
    [下载次数:3 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:15 ]
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