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[Objective] The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Method] Cell division was synchronized by the pepripheral blood lymphocyte culture and the excessive dosage of thymine deoxyribonucleoside, and R-band of Cervus nippon hortulorum chromosomes was also analyzed by RBG-banding technique. [Result] The number of haploid chromosome banding increased to 400. The R-band of No.1, No.2, No.3, No.4, chromosome X and Y were almost just opposite to the high-resolution G band of them. The terminal of chromosomes except No.21, No.24 and No.28 were all positive deeply stained. [Conclusion] R-band of Cervus nippon hortulorum chromosomes can be manifested by RBG-binding technique.
2009年Z4期 v.1 1-3页 [查看摘要][在线阅读][下载 220K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：68 ] [Objective] To study the growth-development of Boer goats from embryo transplant.[Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK,the weight of Boer goats at birth and at the age of 1,3 and 6 months as well as the body size at the age of 3 and 6 months were investigated in this study,while their health conditions were also observed and recorded.[Result] Under the normal feeding conditions,Boer goats from embryo transplant developed well at early stage,and their meat-using body type were also obvious.The average birth weight of bucks was(4.25±0.95) kg,and that of does was(3.74±0.10) kg.The average weight of bucks at the age of 6 months was(31.90±0.74) kg and that of was(25.90±0.67) kg.The daily gains of bucks and ewes from birth to 6 months of age were 153.64 and 123.11 g respectively,while their body length,body height and chest circumference increased obviously,and there was no significant difference in body weight or body size between them and natural breeding goats.[Conclusion] The study lays a foundation for further studies on the breeding and utilization of Boer goats from embryo transplant at early stage.
2009年Z4期 v.1 4-5+9页 [查看摘要][在线阅读][下载 84K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：53 ] -
[Objective] To evaluate lactation performance of primiparous Nubian ewes and Yunling Black ewes.[Method] Healthy primiparous Nubian ewes(20 ewes lambing twins and 19 ewes lambing single) and Yunling Black ewes(4 ewes lambing twins and 15 ewes lambing single) were used to study the effects of breeds and litter size on lactation performance.[Result] The total milk yield of the Nubian ewes lambing twins was 14.81% higher than that of the Nubian ewes lambing single(P<0.05) and 22.93% higher than that of the Yunling Black ewes lambing twins(P<0.05).The total milk yield of the Yunling Black ewes lambing twins was 32.80% higher than that of the Yunling Black ewes lambing single(P<0.05),but the total milk yield of the Nubian ewes lambing single was 42.20% higher than that of the Yunling Black ewes lambing single(P<0.05).The Nubian goats and the Yunling Black goats had similar lactation curves.The milk yield gradually increased during the early lactation period(5-15 d post partum),and the lactation peak lasted for 20 d(15-35 d post partum).Then the milk yield gradually decreased.The predicted values showed the milk yield of the ewes may rapidly decrease during 80-120 d post partum.Until Day 70 post partum,the Nubian ewes lambing twins had lost 3.06% more body weight than those lambing single had(P<0.05);the Yunling Black ewes lambing twins had lost 0.89% more body weight than those lambing single had(P<0.05).The Nubian ewes lambing lost 3.22%-5.39% more body weight than those with the same litter size did(P<0.05).[Conclusion] The regular pattern of primiparous lactation is similar between the Nubian ewes and the Yunling Black ewes,and their lactation performance is closely related to breeds and litter size.
2009年Z4期 v.1 6-9页 [查看摘要][在线阅读][下载 160K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：58 ] [Objective] To find the rule of 5-hydroxytryptamine(5-HT) synthesis and metabolism in peripheral blood of suckling piglets with aging.[Method] All of 15 British Large White pigs at 0,5 and 15 d of age were selected as experimental animals,and their serumal concentrations of L-tryptophan(L-Trp),5-HT and 5-hydroxyindoleacetic acid(5-HIAA) in peripheral circulatory system were determined synchronously by reversed-phase high-performance liquid chromatography method with UV.[Result] The concentration of L-Trp was(16.92±1.74) μmol/L at birth,(59.94±10.88) μmol/L at 5 d of age and(70.42±6.48) μmol/L at 15 d of age.The concentration of 5-HT was(12.85±1.79) μmol/L at birth,(4.81±2.05) μmol/L at 5 d of age and(5.49±1.09) μmol/L at 15 d of age.While 5-HIAA,the metabolic product of 5-HT,was not detected at all the samples selected.[Conclusion] The L-Trp concentration of suckling piglets increase significantly with aging(P<0.05).While the concentration of 5-HT and the conversion ratio of L-Trp to 5-HT are both highest at birth(P<0.05) and keep stable at 5 and 15 d of age.
2009年Z4期 v.1 10-12+15页 [查看摘要][在线阅读][下载 104K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：55 ] -
[Objective] The research was aimed to provide the basis for the development and utilization of Guide black-fur sheep and its disease diagnosis and prevention.[Method] Blood samples were collected from 15 healthy adult Guide black-fur sheep in Black-Fur Sheep Farm of Guinan County in Qinghai Province.Eighteen blood indices belonging to three classes,erythrocyte,leukocyte and platelet,were determined.[Result] The blood indices of the Guide black-fur sheep were as follows:the white blood cell count,(7.52±2.72)×109/L;the red blood cell count,(13.04±2.81)×1012/L;the mean corpuscular hemoglobin,(85.36±27.05) g/L;the mean corpuscular volume,(37.22±1.93) fL;the mean corpuscular hemoglobin concentration,(13.19±0.96) pg;the platelet count,(370.67±139.60)×109/L;the lymphocyte absolute count,(14.11±9.36)×109/L;the absolute count of intermediate cell,(0.79±0.46)×109/L;and the absolute neutrophil count,(0.81±0.71)×109/L.[Conclusion] The research lays the foundation for further protecting the genetic resources of the Guide black-fur sheep and improving their production performance.
2009年Z4期 v.1 13-15页 [查看摘要][在线阅读][下载 81K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：55 ] [Objective] To study the effects of gamma-aminobutyric acid(GABA) on performance of lactating sows during heat stress.[Method] A total of 14 sows at the same parity and with close expected date of childbirth and similar body weight were randomly divided into control group and experimental group.They were fed a common basal diet and a GABA(300 mg/kg) supplementary diet,respectively.The trial lasted for 21 d.[Result] The daily feed intake,lactation yield and average daily gain of piglets in the experimental group were increased by 9.4%,28.5% and 10.7%,respectively.The backfat of lactating sows was decreased less,and the same with the weaning-oestrus interval.The survival ratio of piglets was increased by 4.5%.Compared with the control group,the content of lactose and fat increased significantly,but other components almost did not change.[Conclusion] Supplementing GABA in diet can improve the performance of lactating sows and promote the growth of piglets effectively.
2009年Z4期 v.1 16-17页 [查看摘要][在线阅读][下载 65K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：46 ] -
[Objective] To explore the growth characteristics of domestic and introduced alfalfa varieties and provide scientific guidance for the introduction of superior alfalfa varieties.[Method] A total of 17 domestic and introduced alfalfa varieties were selected.Through a three-year comparison of varieties,their biological characters,plant height,branch density,fresh forage yield,leaf stem ratio and other traits were detected and analyzed.[Result] All the tested alfalfa went through the cold winter between 2006 and 2008 and could finish the reproductive cycle.The forage yield per plant and the plant height of the domestic alfalfa varieties were the highest,but their branching ability and the leaf stem ratio were lower.The European alfalfa varieties had strong branching capability,and the leaf yield of the American alfalfa varieties was the highest.[Conclusion] The domestic alfalfa varieties have superior performances,while the introduced alfalfa varieties have better qualities.
2009年Z4期 v.1 18-21+28页 [查看摘要][在线阅读][下载 115K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：62 ] Porcine transmissible gastroenteritis(TGE) caused by corona virus is an acute,highly contagious,and intestinal infectious disease.TGE is a common disease in intensive pig farms and prevalent throughout the world.Disease in affected pigs is characterized by diarrhea,vomiting and dehydration.In the present article,the pathogen,epidemiology,diagnosis and treatment were elaborated,so as to effectively prevent TGE and provide a reference for treatment.
2009年Z4期 v.1 22-28页 [查看摘要][在线阅读][下载 334K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：43 ] -
[Objective] The aim of this study was to provide the reference for the artificial breeding of aquatic animals.[Method] Amoeba discoides and Trichodina were treated by neutral red solution with different concentrations to observe the physiological activities of organelles in their death processes.Effects of neutral red solution on the growth of common aquatic animals such as Paramecium caudatum,Euglena viridis and Brachionus plicatilis were analyzed,and the specific lethal mechanism of trace neutral red to A.discoides and Trichodina was also preliminarily studied.[Result] The neutral red solution at a concentration of 0.5 mg/L damaged the physiological function of contractile vacuole in Trichodina and also had the specific lethal effect on Trichodina,but it had no effect on the growth and reproduction of non-parasitic protozoa and B.plicatilis.Neutral red solution with certain concentration led to disorder of the physiological functions of A.discoides,such as assimilation and rejection,which was a main factor that caused the death of A.discoides.[Conclusion] With the advantages such as targeting,safety and easiness to be oxidized and decomposed,neutral red is an ideal drug for treating the diseases caused by A.discoides and Trichodina,and its suitable concentration is 0.5 mg/L.
2009年Z4期 v.1 29-32+36页 [查看摘要][在线阅读][下载 445K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：54 ] [Objective] This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate(A/Chicken/Hebei/WD/98,abbreviated as WD98) by comparing with other reference strains.[Method] Eight complete genes were amplified by RT-PCR and sequenced.The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains.[Result] The whole genomic sequence of WD98 strain was 91.1%-95.8% homologous to that of seven reference strains tested.This isolate shared the highest homology(95.8%) to D/HK/Y280/97 and the lowest homology(91.1%) to C/Pak/2/99.The HA cleavage site of the WD98 strain was R-S-S-R↓G,and the 226th amino acid at receptor-binding site was Gln.[Conclusion] WD98 strain belongs to mildly pathogenic avian influenza virus and may not infect human.The genetic relationship is the closest between A/Chicken/Hebei/WD/98 and A/duck/HongKong/Y280/97,both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line.And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line.
2009年Z4期 v.1 33-36页 [查看摘要][在线阅读][下载 224K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：66 ] -
The problems of spatial layout in livestock and poultry farms were discussed,and the development status of the planning evaluation on the spatial layout in recent years was systematically reviewed.The research progress in planning evaluation systems and methods was mainly introduced.And some opinions were proposed to solve these problems.
2009年Z4期 v.1 37-41+46页 [查看摘要][在线阅读][下载 138K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：43 ] According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2(NC001718) available in GenBank(NC_001718),a pair of specific primer was designed,and the target fragment of 431 bp was obtained by PCR amplification.The products were ligated with pMD18-T vector and then transformed into bacteria DH5α for recombinant plasmid extraction.After PCR identification and sequencing,recombinant plasmid was used as a standard template to establish the standard curve of SYBR Green Ⅰ fluorescence quantitative PCR.Sensitivity test,specificity test and repeatability test were also determined.The results indicated that there was a good linear relationship between threshold cycle of the standard curve and template concentration,R2=0.997 6.Tm ranged from 82.3 to 82.9 ℃,while the sensitivity was 72.1 copies/μl with good specificity and repeatability.The developed SYBR Green Ⅰ real-time quantitative PCR method to detect PPV VP2 gene laid the basis for further studies on pathogenesis,early clinical diagnosis of this virus and quantitative analysis of PPV infection.
2009年Z4期 v.1 42-46页 [查看摘要][在线阅读][下载 284K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：66 ] -
<正>1.Requirement for Articles The journal mainly publishes original research papers, research notes, letters and reports about new technology on animal husbandry, veterinary medicine, feed science and other related fields. These articles can refer to basic theory,
2009年Z4期 v.1 47页 [查看摘要][在线阅读][下载 286K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：65 ] <正>Animal Husbandry and Feed Science is published to introduce the research achievements of Animal husbandry and vet erinary workers to the world,enhance their chances to participate in international academic exchange,and promote development of animal husbandry and veterinary.In 2009,USA-China Science & Culture Media Co.(Cranston,USA)
2009年Z4期 v.1 48页 [查看摘要][在线阅读][下载 228K] [下载次数：0 ] |[网刊下载次数：0 ] |[引用频次：0 ] |[阅读次数：55 ]