Basic Veterinary Research

  • Expression,Purification and Activity Detection of Structural Protein VP1 of Foot-and-Mouth Disease Virus Serotype A

    LU Qing-xia;LIU Chang;XING Guang-xu;HAO Hui-fang;JIN Qian-yue;GUO Guan-peng;WANG Fang-yu;YANG Su-zhen;YANG Ji-fei;LIU Yun-chao;DENG Rui-guang;ZHANG Gai-ping;

    The paper was to obtain the VP1 protein of FMDV serotype A with high activity. With recombinant plasmid pMD19A-T-vp1 as the template,vp1 gene fragment amplified by PCR was connected into prokaryotic expression vector pET28 a to construct recombinant plasmid pET-A-vp1.The E. coli BL21( DE3) strain containing recombinant plasmid pET-A-vp1 were induced by IPTG. SDS-PAGE showed that VP1 protein was expressed in the form of inclusion body,and its molecular weight was about 29 ku. Based on the optimizing IPTG concentration and expression time,the largest expression of VP1 protein was induced by 0. 3 mmol /L IPTG for 6 h at 37 ℃. Western-Blot analysis indicated that the expression of VP1 protein could be specifically recognized by positive serum of FMDV serotype A. ELISA test showed that VP1 inclusion body protein had high activity after purification by washing and renaturation by urea concentration gradient dialysis.

    2013年Z1期 v.5 205-209+226页 [查看摘要][在线阅读][下载 374K]
    [下载次数:30 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:14 ]
  • Relationship between Tumor Lesion of Kidney Tissue and Expression of HSP60 in Chickens Infected by MD

    LI Yu-bao;LI Juan;WU Xiao-dong;LIU Yu-tian;ZHAO Yong-gang;ZHANG Yong-qiang;REN Wei-jie;HAN Xiu-ju;WANG Zhi-liang;LIU Si-dang;

    [Objective] The paper was to investigate the relationship between pathological lesion and transcription,expression and distribution of heat shock protein 60( HSP60) in kidney of chickens infected by Marek's disease virus( MDV). [Method] Tumor animal model was successfully established with chickens infected by MDV. Real-time RT-PCR,histopathological and immunohistochemistrical methods were used to detect the pathological lesion,transcription level,expression level and distribution of HSP60 in the kidney. [Result]Obvious pathological changes appeared in kidney tissue of chicken after injection of MDV for 21 d. HSP60 was mainly distributed in the cytoplasm of tumor cell and interstitial macrophages.The expression of HSP60 in challenge group was always higher than blank control group and vaccine control group,and the difference reached extremely significant level from 28 to 86 day-ages( P <0. 01); the expression of HSP60 in challenge group at 28 day-age was about 8. 608 and 12. 752 times of blank control group and vaccine control group,respectively. The mRNA transcription levels of HSP60 in challenge group showed a downward parabola during 7 to 42 day-ages; the mRNA transcription level of HSP60 in challenge group was rising in early stage( 7- 21 d),and then gradually dropped; it reached the peak value at 21 d,being 1. 222 and 1. 179 times of blank control group and vaccine control group,respectively.The mRNA transcription level of HSP60 was higher than two control groups throughout the entire experiment,and the difference reached extremely significant level from 14 to 28 day-ages( P <0. 01). [Conclusion] HSP60 expression in kidney tissue and its mRNA transcription level can reflect MDV infection,tumor formation and development progress,with close relationship with pathogenesis of tumor diseases,which is expected to be used as one of the determination indicators for diagnosis and morbidity process of tumor diseases in chicken.

    2013年Z1期 v.5 210-215页 [查看摘要][在线阅读][下载 504K]
    [下载次数:8 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:19 ]

Feeding & Management

  • Finish Effect of Different Feeding Patterns on Weaned Lamb

    BAI Yun-feng;TU Yuan-lu;BAI Yi-la;GAO Li-peng;YAN Shao-hua;LIU Jian;

    Finish effect of four feeding patterns on weaned lamb was studied in the paper. The results showed that the performance of growing lambs by agricultural byproduct residual forage was superior to other feeding methods; too high proportion of straw in diet was unfavorable for finish lamb,and corn straw should be combined with complete feed to get better feeding effect.

    2013年Z1期 v.5 216-218+239页 [查看摘要][在线阅读][下载 97K]
    [下载次数:7 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:17 ]

Grassland Resources

  • Biological Characteristics of Exorista civilis,A Dominant Parasitoid of Loxostege sticticalis L.

    XIANG Hong-yan;LIU Ai-ping;GAO Shu-jing;XU Lin-bo;LIU Chang-zhong;MIAO Li-mei;KANG Ai-guo;WANG Jian-mei;

    Exorista civilis Rond.( Diptera: Tachinidae) is an important parasitoid of Loxostege sticticalis L.( Lepidoptera: Pyralidae) and other lepidopterous pests. The biological characteristics of E. civilis were observed in the laboratory with L. sticticalis as host. The adults emerged mainly during the daytime from 08:00 am to 10: 00 am and 14:00 pm to 16:00 pm,and males emerged usually earlier than females,with the sex ratio of 1.26:1( ♀/♂). The average fecundity of female( number of eggs laid by one female) was 159. 8 eggs,and the spawning duration lasted 16. 6 d on average; the first spawning peak appeared on the fourth day after spawning. The longevity of adults was the longest at constant temperature of( 23 ±1) ℃. Supplement of sugary substances could significantly prolong the longevity of adults,especially feeding20% hydromel could extend the longevity to 44 d. The emergence rate of E. civilis significantly decreased with an increase in storage time of L. sticticalis cocoon,but there was no further significant decrease after 110 d.

    2013年Z1期 v.5 219-223+235页 [查看摘要][在线阅读][下载 297K]
    [下载次数:16 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:17 ]

Animal Genetics&Breeding

  • Growth Performance of Different Cross Combinations of Quality Lean Type Pig

    SHEN Xue-lin;DUAN Yong-bang;

    Ten healthy weaning piglets of Landrace × Yorkshire( LY) in good body condition with body weight of( 24 ±1. 27) kg were selected,and ten weaning piglets of Berkshire × LY( BLY) and Duroc × LY( DLY) with similar situation were also selected respectively to study the growth performance of different cross combinations. Three groups of experimental pigs were bred with conventional diet. The results showed that the average dry matter intakes of combinations of BLY and DLY were slightly decreased by 4. 97% and 5. 47% compared with the combination of LY,while average daily gain was significantly increased by 3. 13% and 1. 14%,and feed-weight ratio was decreased by 7. 94% and 6. 35%,respectively; economic benefit per pig was increased by 0. 61 and 0. 41 yuan,respectively. The combinations of BLY and DLY have better growth performance,which can be promoted in the production.

    2013年Z1期 v.5 224-226页 [查看摘要][在线阅读][下载 174K]
    [下载次数:6 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:16 ]
  • Construction and Expression of Recombinant Ghrelin Plasmid and Effects on Growth Performance and Gastric Acid Secretion of Rats

    DU Gai-mei;JIANG Jia-jin;YAN Wen-mei;HU Zhi-hua;LUO Bi-ping;ZHANG Yu-hong;ZHANG Miao;LI Liu-an;LIU Mao-jun;

    The paper was to study construction and expression of rGhrelin( pcDNA3- Ghrelin) and its effect on growth performance and gastric acid secretion of rats. Ghrelin amplified from gastric mucosa of weaned piglets was cloned into the expression vector pcDNA3 to get recombinant plasmid pcDNA3- Ghrelin. Twelve weaning rats were randomly divided into two groups,six rats each group. The rats in each treatment group were individually injected with 100μg of naked plasmid pcDNA3- Ghrelin,and the rats in control group were injected with empty plasmid. The weights and feed consumption of rats were measured after injection for 7,14 and 29 d,respectively. The rats were sacrificed at the end of the experiment,and their stomach was separated and weighed,the pH value of gastric juice was measured as well. The results showed that the average daily gain of rats at7 and 29 d were significantly higher than that in control group,respectively( P <0. 05),and feed consumption did not have significant changes; the feed meat ratio of rats in the treatment groups was significantly lower than that in control group( P <0. 05); the gastric relative weight and gastric weight did not change significantly,while the pH value of gastric juice of rats in treatment groups was significantly lower than that in control group( P<0. 05). This indicated that after transfected expression of muscle tissue,ghrelin played an important regulatory role in growth and gastric acid secretion of rats.

    2013年Z1期 v.5 227-231页 [查看摘要][在线阅读][下载 471K]
    [下载次数:7 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:162 ]
  • Correlation of Evaluation Methods on Boar Semen Quality

    WU Cai-hong;YAO Jing;ZHANG Bin;FU Hai-ke;YANG Zhi-fen;LI Kai;SHENG Qiang;

    To explore the correlation of evaluation methods on boar semen quality,the motility,PI staining,hypotonic swelling test,Rh123 staining and FITC- PNA staining of boar sperm were checked in the study,and its correlation with IVF was statistically calculated. The results showed that various indicators had strong linear correlation with each other,and the correlation degree between acrosome integrity and IVF rate was the highest,indicating that acrosome integrity was superior to other indicators in predicting IVF rate.

    2013年Z1期 v.5 232-235页 [查看摘要][在线阅读][下载 227K]
    [下载次数:13 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:14 ]
  • Effects of Hybridizing Berkshire Boar with Landrace × Yorkshire Sow on the Carcass Traits and Meat Quality of Hybrid Progeny

    SHEN Xue-lin;LI Ai-ping;PU Xue-dong;

    [Objective] The research aimed to provide theoretical basis for further popularization and application of Berkshire Pig in Southeast Guizhou. [Method]The carcass traits and meat quality of Berkshire( B) × Landrace( L) × Yorkshire( Y),Duroc( D) × Landrace( L) × Yorkshire( Y)and Landrace( L) × Yorkshire( Y) were determined by slaughter test. And the application effect of Berkshire in the minority areas was analyzed.[Result]From the carcass traits,the hybridized pig of Berkshire( B) ×Landrace( L) ×Yorkshire( Y) had higher slaughter rate and lower lean meat percentage,having extremely significant difference with Landrace( L) × Yorkshire( Y) and significant difference with Duroc( D) × Landrace( L) ×Yorkshire( Y). The meat indices of Berkshire( B) × Landrace( L) × Yorkshire( Y) were better than that of Landrace( L) × Yorkshire( Y) and Duroc( D) ×Landrace( L) ×Yorkshire( Y). The eye muscle area of Berkshire( B) ×Landrace( L) ×Yorkshire( Y) had extremely significant difference with Landrace( L) × Yorkshire( Y) and significant difference with Duroc( D) × Landrace( L) × Yorkshire( Y). The meat color,marbling and cooking percentage and other meat indices were similar with that of Jianhe White Xiang Pig,Nayong Nuogu Pig and Cola Pig. [Conclusion] Berkshire( B) ×Landrace( L) × Yorkshire( Y) was more suiTab. for residents in the minority mountain areas,so it was worthy to be popularized.

    2013年Z1期 v.5 236-239页 [查看摘要][在线阅读][下载 110K]
    [下载次数:12 ] |[网刊下载次数:0 ] |[引用频次:2 ] |[阅读次数:16 ]

Animal Nutrition

  • Effect of Feed Protein Levels on Growth and Feed Utilization of Juvenile Lateolabrax japonicas

    LIN Xing;

    To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabrax japonicus,fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containing 35.0%,37.5%,40.0%,42.5%and 45.0%protein,respectively.Juvenile L.japonicus with the initial average body weight of(84.81±0.92)g were fed for 75 d to determine the best protein level for juvenile L.japonicus.The results showed that the relative weight gain rate,specific growth rate and feed conversion ratio in the group containing 39.85% protein were extremely higher than those in the groups containing 34.76% and 37.54%protein (P<0.01),but had no significant difference with that in the group containing 42.34%protein(P>0.05).Protein efficiency ratio in the group containing 39.85%protein was also significantly higher than those in the groups containing 34.76%,42.34%and 45.03%protein(P<0.05),while had no significant difference with that in the group containing 37.54% protein (P>0.05).In this experiment,based on quadratic model regression analysis of specific growth rate and protein efficiency ratio,growth performance and feed utilization rate of L.japonicus was the best when the protein content of feed was from 38.87%to 41.50%.

    2013年Z1期 v.5 240-243+248页 [查看摘要][在线阅读][下载 189K]
    [下载次数:27 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:169 ]
  • Feed Production for Tenebrio molitor L. by Fermentation of Corn Stalks

    XU Shi-cai;XI Zeng-jun;SHEN Xue-jian;AI Jia-le;

    In order to reduce the economic costs for breeding Tenebrio molitor L. and increase the utilization rate of corn stalk,with corn,wheat bran and corn stalk as the raw materials,13 different compound feed were designed by Minitab( R) 15. 1. 0. 0. The design was then divided into two groups based on addition or without addition of vegetable leaves to study the effects of compound feed and vegetable leaves on growth and development of T. molitor. The results showed that body weight,duration,crude protein content and pupation rate of larvae and emergence rate of adults were significantly affected by compound feed and vegetable leaves( P <0. 05). The larvae bred with feed 5 had the largest body weight,with the shortest duration; the larvae bred with feed 3 had the highest pupation rate; the larvae bred with feed 7 had the highest crude protein content;the adult bred with feed 9 had the largest emergence rate. Overall breeding effect of feed 7 + vegetable leaves was better than any other groups,with ideal indicators,higher utilization rate and lower breeding cost. Its formula was w( wheat bran) = 33. 33%,w( corn) = 33. 33%,w( corn stalks) = 33. 33%. Therefore,the growth of T. molitor was significantly affected by feed types,and feed 7 + vegetable leaves could be promoted in the production.

    2013年Z1期 v.5 244-248页 [查看摘要][在线阅读][下载 241K]
    [下载次数:83 ] |[网刊下载次数:0 ] |[引用频次:1 ] |[阅读次数:120 ]

  • Instructions for Authors

    <正>1.Requirement for Articles The journal mainly publishes original research papers,research notes,letters and reports about new technology on animal husbandry,veterinary medicine,feed science and other related fields.These articles can refer to basic theory,applied research and high-tech,etc.A manuscript must offer detailed document and reliable data.The innovative idea should be described concisely and definitely.A review should

    2013年Z1期 v.5 249页 [查看摘要][在线阅读][下载 671K]
    [下载次数:2 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:20 ]
  • About Animal Husbandry and Feed Science

    <正>Animal Husbandry and Feed Science is published to introduce the research achievements of animal husbandry and veterinary workers to the world,enhance their chances to participate in international academic exchange,and promote development of animal husbandry and veterinary.In 2009,Wu Chu(USA-China)Science&Culture Media Co.(Cranston,USA)and Anhui Wuchu Science,Technology and Culture Communication Co.,Ltd(Hefei,China)issued the

    2013年Z1期 v.5 250页 [查看摘要][在线阅读][下载 504K]
    [下载次数:5 ] |[网刊下载次数:0 ] |[引用频次:0 ] |[阅读次数:13 ]
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